SB-273005, an antagonist of αvβ3 integrin, reduces the production of Th2 cells and cytokine IL-10 in pregnant mice
Pregnancy is connected with complex immunoreactions. In our study, the result of Senate bill-273005, an antagonist of avß3 integrin, around the alterations of T assistant (Th) cells as well as their derived cytokines that occur while pregnant was investigated in rodents. Five non-pregnant rodents were utilised like a negative control. Rodents were impregnated by co-housing females and males in a ratio of two:1 overnight and pregnancy was confirmed by the look of vaginal plugs the next morning. First Day (D1) pregnant rodents were at random split into two groups (n=20) and were administered either dimethylsulfoxide (mock treatment) or Senate bill-273005 (3 mg/kg) by gavage at D3, D4 and D5. At D8, the amount of Th1 and Th2 cells and interleukin (IL)-2 and IL-10 within the spleen and peripheral bloodstream were determined using flow cytometry and enzyme-linked immunosorbent assay. Pregnancy considerably SB273005 elevated the number of Th2:Th1 cells within the spleen in contrast to that in non-pregnant rodents (P<0.01). However, this increase was significantly reduced by SB-273005 (P<0.001). Furthermore, whilst pregnancy decreased Th1 cell-produced IL-2 levels and increased Th2 cell-derived IL-10 levels, SB-273005 reversed both processes (P<0.05 for IL-2 P<0.01 for IL-10). The results from the present study demonstrated that pregnancy induces changes in the spleen, including a reduction of IL-2 and an increase in IL-10 production by Th1 and Th2 cells, respectively, as well as an upregulation of the Th2:Th1 ratio in the spleen. These immunological changes are reversed by SB-273005, indicating an important role for avß3 integrin in mediating these immunological alterations.